win2003 iis6.0 NET2.0的Server Application Unavailable的问题

Boolean throwOnFileNotFound, Boolean forIntrospection)
at System.Reflection.Assembly.InternalLoad(AssemblyName assemblyRef, Evidence assemblySecurity, StackCrawlMark& stackMark, Boolean forIntrospection)
at System.Reflection.Assembly.InternalLoad(String assemblyString, Evidence assemblySecurity, StackCrawlMark& stackMark, Boolean forIntrospection)
at System.Activator.CreateInstance(String assemblyName, String typeName, Boolean ignoreCase, BindingFlags bindingAttr, Binder binder, Object[] args, CultureInfo culture, Object[] activationAttributes, Evidence securityInfo, StackCrawlMark& stackMark)
at System.Activator.CreateInstance(String assemblyName, String typeName)
at System.AppDomain.CreateInstance(String assemblyName, String typeName)
at System.AppDomain.CreateInstance(String assemblyName, String typeName)
at System.Web.Hosting.ApplicationManager.CreateAppDomainWithHostingEnvironment(String appId, IApplicationHost appHost, HostingEnvironmentParameters hostingParameters)
at System.Web.Hosting.ApplicationManager.CreateAppDomainWithHostingEnvironmentAndReportErrors(String appId, IApplicationHost appHost, HostingEnvironmentParameters hostingParameters)
有关更多信息，请参阅在 http://go.microsoft.com/fwlink/events.asp 的帮助和支持
怎么办，怎么才可以运行这个网页（这个网页在我有VS2005的机器上运行调试是都可以的！）
----------------------------------------------------------------------
看看IIS是不是关了，如果开着就重新配置一下虚拟目录
--------------------------------------------------------
怎么看有没有关，怎么配置虚拟目录，我以前没弄过！
--------------------------------------------------------
服务器是2003，IIS是V6.0
--------------------------------------------------------
我也遇到这样的问题,那位高人指点一下啊!
--------------------------------------------------------
up
--------------------------------------------------------
搞定虚拟目录指向的文件夹要加User组
--------------------------------------------------------
我是菜鸟，不知道我的回答是否多余！？
如果装过IIS，默认应该是打开的，除非改过服务。
去控制面板-〉性能和维护-〉管理工具-〉Internet信息服务
就可以看到了，还可以设置虚拟目录
解决办法:在该web sites的virtual directory下,将Permissions设置一下,里面应包含
ASP.NET
IWAM_MachineName
Local_Service
Network_Service
System
而且权限要设置为write & read.